MTORC2 contributes to the metabolic reprogramming in EGFR tyrosine-kinase inhibitor resistant cells in non-small cell lung cancer. (10th October 2018)

• Record Type: Journal Article
• Authors: Chiang, Chun-Te; Demetriou, Alexandra N.; Ung, Nolan; Choudhury, Niharika; Ghaffarian, Kimya; Ruderman, Daniel L.; Mumenthaler, Shannon M.
• Is Part Of: Cancer letters. Volume 434(2018)
• Page Start: 152
• Page End: 159
• Publication Date: 2018-10-10
• Subjects: NSCLC -- EGFR TKI resistance -- Glucose metabolism -- mTORC2; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2018.07.025
• Languages: English
• ISSNs: 0304-3835

Abstract

Abstract: Non-small cell lung cancer (NSCLC) patients with activating EGFR mutations are often successfully treated with EGFR tyrosine kinase inhibitor (TKI) such as erlotinib; however, treatment resistance inevitably occurs. Given tumor metabolism of glucose and therapeutic response are intimately linked, we explored the metabolic differences between isogenic erlotinib-sensitive and -resistant NSCLC cell lines. We discovered that the growth of erlotinib-resistant cells is more sensitive to glucose deprivation. Seahorse metabolic assay revealed erlotinib-resistant cells have lower spare respiratory capacity (SRC), an indicator of metabolic flexibility, compared to erlotinib-sensitive cells. Additionally, we found downstream components of mTORC2 signaling to be phosphorylated in erlotinib-resistant cells. Knockdown of an mTORC2 component, Rictor, enhanced the SRC and rescued the growth rate of erlotinib-resistant cells during glucose deprivation. Among NSCLCs with activating EGFR mutations, gene sets involved in glucose metabolism were enriched in patients with high expression of p-NDGR1, a readout of mTORC2 activity. Furthermore, overall survival was negatively correlated with p-NDRG1. Our work uncovers a link between mTORC2 and metabolic reprogramming in EGFR TKI-resistant cells and highlights the significance of mTORC2 in the progression of EGFR -mutated NSCLC. Highlights: The growth rate of erlotinib-resistant cells is sensitive to glucose deprivation. Erlotinib-resistant cells have diminished spare respiratory capacity. mTORC2 contributes to metabolic reprogramming in erlotinib-resistant cells. mTORC2 activation correlates with clinical outcome of EGFR -mutated NSCLC patients.