Understanding the elusive protein corona of thermoresponsive nanogels. (9th October 2018)

• Record Type: Journal Article
• Authors: Miceli, Enrico; Kuropka, Benno; Rosenauer, Christine; Osorio Blanco, Ernesto R; Theune, Loryn E; Kar, Mrityunjoy; Weise, Christoph; Morsbach, Svenja; Freund, Christian; Calderón, Marcelo
• Is Part Of: Nanomedicine. Volume 13:Number 20(2018)
• Publication Date: 2018-10-09
• Subjects: albumin -- apolipoprotein B-100 -- dendritic polyglycerol -- pNIPAM -- pNIPMAM -- protein corona -- proteomics -- thermoresponsive nanogels; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2018-0217
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: We analyzed the protein corona of thermoresponsive, poly( N -isopropylacrylamide)- or poly( N -isopropylmethacrylamide)-based nanogels.Materials & methods: Traces of protein corona detected after incubation in human serum were characterized by proteomics and dynamic light scattering in undiluted serum.Results: Apolipoprotein B-100 and albumin were the main components of the protein coronae. For dendritic polyglycerol-poly( N -isopropylacrylamide) nanogels at 37°C, an increase in adsorbed immunoglobulin light chains was detected, followed by partially reversible nanogel aggregation. All nanogels in their hydrophilic state are colloidally stable in serum and bear a dysopsonin-rich protein corona.Conclusion: We observed strong changes in NG stability upon slight alterations in the composition of the protein coronae according to nanogel solvation state. Nanogels in their hydrophilic state possess safe protein coronae.