Magnetic extraction of toxin binding liposomes; a method to ameliorate drug toxicity? Preliminary in vitro/in vivo study. (20th November 2018)

• Record Type: Journal Article
• Authors: Cave, Grant; Harvey, Martyn; Sleigh, Jamie; Kanamala, Manju; Wu, Zimei
• Is Part Of: Nanomedicine. Volume 13:Number 24(2018)
• Page Start: 3083
• Page End: 3089
• Publication Date: 2018-11-20
• Subjects: amitriptyline -- antidote -- dialysis -- liposomes -- magnetite -- poisoning; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2018-0198
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: Removal of a toxin from the body once absorbed is usually not possible. We describe the use of magnetite containing pH gradient 'MagnepH' liposomes to overcome limitations preventing removal.Methods: MagnepH liposomes were added to albumin solution containing amitriptyline and dosed intravenously in rats prior to amitriptyline injection. Albumin solution or drawn blood was exposed to a magnet and sampled.Results: One third of amitriptyline was extracted in vitro . In vivo amitriptyline concentrations were 1830 nmol/l (controls) and 10870 nmol/l (MagnepH; n = 2). Amitriptyline extraction increased from 0.6% (control) to 10.4% (MagnepH; 95% CI for difference 2.0–17.6%).Conclusion: MagnepH liposomes sequestered amitriptyline and could then be extracted. This method has potential to ameliorate limitations to extracorporeal removal of toxins in poisoning.