YAP/TAZ mechano-transduction as the underlying mechanism of neuronal differentiation induced by reduced graphene oxide. (19th November 2018)

• Record Type: Journal Article
• Authors: Catanesi, M; Panella, G; Benedetti, E; Fioravanti, G; Perrozzi, F; Ottaviano, L; Leandro, L Di; Ardini, M; Giansanti, F; d'Angelo, M; Castelli, V; Angelucci, F; Ippoliti, R; Cimini, A
• Is Part Of: Nanomedicine. Volume 13:Number 24(2018)
• Page Start: 3091
• Page End: 3106
• Publication Date: 2018-11-19
• Subjects: biomaterials -- matrix stiffness -- mechanotrasduction -- nanomaterials -- neuroblastoma -- neuronal differentiation -- reduced graphene oxide -- SmPrxI -- surface topography -- YAP/TAZ; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2018-0269
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: The aim of this work is the dissection of the molecular pathways underlying the differentiation effect of reduced graphene oxide (GO) materials in the absence of differentiation agents.Materials & methods: Reduced GO is obtained either by drop casting method and heat-treated or biological reduction by the interaction between GO and wtPrxI. Cells were grown on both materials and the differentiation process studied by immunological and morphological detection.Results & conclusion: The results obtained indicate that both reduction methods of GO can determine the modulation of pathway involved in mechano-transduction and differentiation, by affecting YAP/TAZ localization outside the nuclei and increasing neuronal differentiation markers. This suggests that the mechano-transduction pathways are responsible for the differentiation process.