Assessing gene function in human B cells: CRISPR/Cas9‐based gene editing and mRNA‐based gene expression in healthy and tumor cells. Issue 8 (22nd May 2022)

• Record Type: Journal Article
• Authors: Nardi, Federica; Pezzella, Laura; Drago, Roberta; Di Rita, Anthea; Simoncelli, Martina; Marotta, Giuseppe; Gozzetti, Alessandro; Bocchia, Monica; Kabanova, Anna
• Is Part Of: European journal of immunology. Volume 52:Issue 8(2022)
• Page Start: 1362
• Page End: 1365
• Publication Date: 2022-05-22
• Subjects: B cells -- Chronic lymphocytic leukemia (CLL) -- CRISPR/Cas9 -- mRNA -- Screening; Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.202149784
• Languages: English
• ISSNs: 0014-2980

Abstract

Abstract : Robust methods for manipulation of human B cells, isolated from healthy donors and patients with B cell disorders, has the potential to significantly accelerate B cell research. Our work describes a step‐by‐step protocol to perform electroporation‐based screening of gene function in B cells through the use of Cas9 ribonuclecomplexes and in vitro produced mRNA.