Mass spectrometry and the cellular surfaceome. Issue 5 (2nd March 2021)

• Record Type: Journal Article
• Authors: Pauwels, Jarne; Fijałkowska, Daria; Eyckerman, Sven; Gevaert, Kris
• Is Part Of: Mass spectrometry reviews. Volume 41:Issue 5(2022)
• Page Start: 804
• Page End: 841
• Publication Date: 2021-03-02
• Subjects: enrichment strategies -- LC‐MS/MS -- plasma membrane proteins -- proteomics -- surfaceome; Mass spectrometry -- Periodicals; 543
• DOI: 10.1002/mas.21690
• Languages: English
• ISSNs: 0277-7037

Abstract

Abstract: The collection of exposed plasma membrane proteins, collectively termed the surfaceome, is involved in multiple vital cellular processes, such as the communication of cells with their surroundings and the regulation of transport across the lipid bilayer. The surfaceome also plays key roles in the immune system by recognizing and presenting antigens, with its possible malfunctioning linked to disease. Surface proteins have long been explored as potential cell markers, disease biomarkers, and therapeutic drug targets. Despite its importance, a detailed study of the surfaceome continues to pose major challenges for mass spectrometry‐driven proteomics due to the inherent biophysical characteristics of surface proteins. Their inefficient extraction from hydrophobic membranes to an aqueous medium and their lower abundance compared to intracellular proteins hamper the analysis of surface proteins, which are therefore usually underrepresented in proteomic datasets. To tackle such problems, several innovative analytical methodologies have been developed. This review aims at providing an extensive overview of the different methods for surfaceome analysis, with respective considerations for downstream mass spectrometry‐based proteomics.