Oral RNAi toxicity assay suggests clathrin heavy chain as a promising molecular target for controlling the 28‐spotted potato ladybird, Henosepilachna vigintioctopunctata. Issue 9 (4th September 2021)

• Record Type: Journal Article
• Authors: Guo, Mujuan; Nanda, Satyabrata; Chen, Shimin; Lü, Jing; Yang, Chunxiao; Liu, Zhuoqi; Guo, Wei; Qiu, Baoli; Zhang, Youjun; Zhou, Xuguo; Pan, Huipeng
• Is Part Of: Pest management science. Volume 78:Issue 9(2022)
• Page Start: 3871
• Page End: 3879
• Publication Date: 2021-09-04
• Subjects: biopesticide -- eco‐friendly pest control -- ladybird pest -- non‐target effect -- RNAi product; Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.6594
• Languages: English
• ISSNs: 1526-498X

Abstract

Abstract: BACKGROUND: Use of RNA interference (RNAi) technology in effective pest management has been explored for decades. Henosepilachna vigintioctopunctata is a major solanaceous crop pest in Asia. In this study, the effects of the RNAi‐mediated silencing of clathrin heavy chain in H. vigintioctopunctata were investigated. RESULTS: Feeding either the in vitro ‐synthesized or the bacterially expressed double‐stranded RNAs (dsRNAs) significantly impaired the normal physiology of H. vigintioctopunctata instars and adults. However, the bacterially expressed ds HvChc caused higher mortality than the in vitro ‐synthesized ones in the larvae and adults. Moreover, on evaluating the potential risk of ds HvChc on Propylea japonica, significant transcriptional effects of ds HvChc1 were observed, while the organismal level effects were not significant. On the contrary, ds HvChc2 did not affect P. japonica at either level. A similar test revealed significant transcriptional effects of ds PjChc1 on H. vigintioctopunctata, while staying ineffective at the organismal levels. Conversely, ds PjChc2 did not affect H. vigintioctopunctata at either level. Importantly, no effect of ds PjChc1 exposure on H. vigintioctopunctata suggested that other factors besides the 21‐nucleotide (nt) matches between sequences were responsible. Finally, ingestion of ds HvmChc1 derived from H. vigintioctomaculata, containing 265‐nt matches with ds HvChc1, caused 100% mortality in H. vigintioctopunctata . CONCLUSIONS: We conclude that (i) species with numerous 21‐nt matches in homologous genes are more likely to be susceptible to dsRNA; (ii) dsRNA can be safely designed to avoid negative effects on non‐target organisms at both transcriptional and organismal levels; (iii) HvChc can be used as an efficient RNAi target gene to effectively manage H. vigintioctopunctata . © 2021 Society of Chemical Industry. Abstract : RNA interference (RNAi) has potential to develop eco‐friendly control strategies against insect pests. We tested double‐stranded RNAs (dsRNAs) as biopesticides against Henosepilachna vigintioctopunctata . We also tested the effect on a closely related non‐target organism. dsRNA can be designed to avoid negative effects on non‐target organisms. HvChc is an effective RNAi target gene for controlling H. vigintioctopunctata .