Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae. Issue 6 (June 2019)

• Record Type: Journal Article
• Authors: Vargas, J.M.; Moreno Mochi, M.P.; Nuñez, J.M.; Cáceres, M.; Mochi, S.; del Campo Moreno, R.; Jure, M.A.
• Is Part Of: Heliyon. Volume 5:Issue 6(2019)
• Publication Date: 2019-06
• Subjects: Epidemiology -- Infectious disease -- Microbiology -- Carbapenemase -- KPC -- Klebsiella pneumoniae -- Multi-clonal; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2019.e01829
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: Carbapenemase-producing Klebsiella pneumoniae (CRKP) are increasingly reported worldwide being necessary the local epidemiological monitoring. Our aim was to characterize the hypermucoviscous CRKP isolates collected in our hospital during a 6 months period. Carriage of the carbapenemase genes ( bla KPC, bla NDM, bla VIM and bla OXA-48 ), extended spectrum β-lactamases ( bla SHV-2, bla CTX-M ) and the virulence genes ( mag A, k2A, rmp A, wab G, uge, all S, ent B, ycf M, kpn, wca G, fim H, mrk D, iut A, iro N, hly and cnf -1) were determined by multiplex-PCR. Genetic relationship among the isolates was performed by PFGE and MLST. A total of 35 isolates were recovered, being the urinary and respiratory tract the most common infection sites (34.2%). The bla KPC-2 gene was present in all the isolates, coexisting with bla CTX-M-2 (45.7%), bla SHV-2 (28.6%), and bla CTX-M-2 / bla SHV-2 (14.3%). The capsular serotype K2 corresponded with 68.6% of the isolates. Virulence factors frequency were variable [adhesins (97.1%), siderophores (94.3%) and phagocytosis resistance ( wab G 48.5%, uge 80% and ycf M 57.1%)]. A total of 10 STs were identified although 40% of them clustered on ST25-CC65, and 17% to ST17. The incidence of KPC-2-producing K. pneumoniae reported by the hospital was 0.290 per 1000 admissions. In summary we described an epidemic scenario of multidrug resistant hypermucoviscous KPC-2 producing ST25 K. pneumoniae in our institution.