Association study of M235T and A‐6G polymorphisms in angiotensinogen gene with risk of developing preeclampsia in Iranian population. (15th May 2019)

• Record Type: Journal Article
• Authors: Alaee, Ehsan; Mirahmadi, Maryam; Ghasemi, Masoumeh; Kashani, Elham; Attar, Marzieh; Shahbazi, Majid
• Is Part Of: Annals of human genetics. Volume 83:Number 6(2019:Nov.)
• Page Start: 418
• Page End: 425
• Publication Date: 2019-05-15
• Subjects: angiotensinogen -- polymorphism -- preeclampsia; Human genetics -- Periodicals; 599.935
• DOI: 10.1111/ahg.12323
• Languages: English
• ISSNs: 0003-4800

Abstract

Abstract: Objective: Preeclampsia (PE) is a life‐threatening complication of pregnancy that accounts for 12% of all maternal deaths worldwide. The aim of this study is to investigate the relationships between the polymorphisms of angiotensinogen (AGT) gene and preeclampsia. Material and methods: In this study, 240 unrelated preeclampsia patients and 178 normotensive women were examined. Genomic DNA was extracted then we assessed M235T(C/T) and A‐6G polymorphisms of the AGT gene. Genotyping of M235T and A‐6G polymorphisms were performed using SSP‐PCR and MS‐PCR, respectively. Results: A significant protective association was observed between A‐6G G allele, A‐6G A/G heterozygote genotype (OR = 0.6, p = 0.007 and OR = 0.6, p = 0.04) against PE. Furthermore, it was shown that two copies of A‐6G A allele would increase PE risk (OR: 0.62, p = 0.04). Our results did not show a significant association for M235T polymorphism and PE. However, the combinations of A‐6G A/A genotype and M235T T/C genotype (OR = 0.4, p = 0.02) and also A‐6G A/G genotype and M235T T/C genotype (OR = 0.5, p = 0.04) in controls represented a significant protective association against PE. Conclusion: According to the existence of significant correlation between two candidate polymorphisms, A‐6G and M235T polymorphisms, with PE disease in our study, they may be considered as valuable factors in susceptibility to PE disease in Iranian women.