LINC01559 accelerates pancreatic cancer cell proliferation and migration through YAP‐mediated pathway. Issue 4 (14th October 2019)

• Record Type: Journal Article
• Authors: Lou, Changjie; Zhao, Juan; Gu, Yuanlong; Li, Qingwei; Tang, Shuli; Wu, Yangjiazi; Tang, Jiebing; Zhang, Chunhui; Li, Zhiwei; Zhang, Yanqiao
• Is Part Of: Journal of cellular physiology. Volume 235:Issue 4(2020:Apr.)
• Page Start: 3928
• Page End: 3938
• Publication Date: 2019-10-14
• Subjects: LINC01559 -- miR‐607 -- pancreatic cancer -- YAP; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.29288
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Pancreatic cancer (PC) is one of the top two most fatal cancers, with the poorest survival rate among all human malignancies. Increasing evidence suggests the involvement of long noncoding RNAs (lncRNAs) in the initiation and progression of various cancers. Herein, we investigated the role of lncRNA LINC01559 in PC. Several online databases indicated that LINC01559 was at a low expression in normal pancreatic tissues but was obviously upregulated in PAAD tissues. Further, our results showed that LINC01559 was stimulated in PC cell lines relative to normal controls. Furthermore, we validated that LINC01559 facilitated PC cell proliferation and migration in vitro. Also, silencing LINC01559 obstructed PC cell growth in vivo. Besides, LINC01559 was revealed to be mainly in the cytoplasm of PC cells and therefore served as a ceRNA of Yes‐associated protein (YAP) in PC cells via sponging miR‐607. Surprisingly, we also proved that LINC01559 could interact with YAP protein, which might hinder YAP phosphorylation and enhance YAP transcriptional activity in PC cells. Furthermore, we demonstrated that YAP was the downstream effector in LINC01559‐regulated PC development. Collectively, our findings unmasked that LINC01559 accelerates PC progression through relying on YAP, providing a new potential target for clinical treatment of patients with PC. Abstract : LINC01559 is highly‐expressed in pancreatic cancer (PC) and its inhibition controls PC progression both in vitro and in vivo. LINC01559 boosts Yes‐associated protein (YAP) expression through competitively binding with miR‐607. LINC001559‐contributed PC progression is mediated by YAP.