Development and optimization of multicolumn chromatography for mAb perfusion processes. Issue 9 (13th May 2021)

• Record Type: Journal Article
• Authors: Gillette, Keith; Bisschops, Marc; Schofield, Mark
• Is Part Of: Journal of chemical technology & biotechnology. Volume 97:Issue 9(2022)
• Page Start: 2290
• Page End: 2296
• Publication Date: 2021-05-13
• Subjects: process intensification -- chromatography -- process development -- industrial biotechnology; Biotechnology -- Periodicals; Chemistry, Technical -- Periodicals; Chemical engineering -- Periodicals; Industries -- Environmental aspects -- Periodicals; 660
• DOI: 10.1002/jctb.6757
• Languages: English
• ISSNs: 0268-2575

Abstract

Abstract: The maturation of the bioprocessing industry is driving the desire to reduce manufacturing costs through process intensification. Here we discuss the motivation for more productive upstream perfusion processes and the implications for downstream purification. The focus of the article is how to optimize the downstream process chromatography steps based on perfusion upstream. A range of different chromatography solutions are explored and their possible impact on the purification process discussed. © 2021 Society of Chemical Industry