Synergistic effects of myo‐inositol and melatonin on cryopreservation of goat spermatozoa. Issue 8 (11th May 2022)

• Record Type: Journal Article
• Authors: Tanhaei Vash, Nima; Nadri, Parisa; Karimi, Akbar
• Is Part Of: Reproduction in domestic animals. Volume 57:Issue 8(2022)
• Page Start: 876
• Page End: 885
• Publication Date: 2022-05-11
• Subjects: cryopreservation -- goat sperm -- in vitro fertilization -- melatonin -- myo‐inositol -- oxidative stress; Animal breeding -- Periodicals; Veterinary pathology -- Periodicals; Livestock -- Breeding -- Periodicals; 636.082
• DOI: 10.1111/rda.14131
• Languages: English
• ISSNs: 0936-6768

Abstract

Abstract: Overproduction of reactive oxygen species (ROS) during sperm cryopreservation has a detrimental effect on sperm parameters. Therefore, the use of antioxidants in the sperm freezing extender can reduce ROS destructive effects. In this study, we investigated whether co‐supplementation of melatonin and myo‐inositol into the semen extender can improve the post‐cryopreservation quality of goat spermatozoa. After the freeze–thawing process, sperm motility, viability, plasma membrane and acrosome intact morphology were improved in the combined myo‐inositol and melatonin group compared to both individual and the control groups ( p < .05). In addition, the mean of sperm ROS, DNA damage and lipid peroxidation were reduced in co‐supplementation of myo‐inositol and melatonin compared to their individual counterparts ( p < .05). Therefore, the synergistic effects of myo‐inositol and melatonin on the cryopreserved spermatozoa are highly likely mediated through the reduction in important factors involved in the sperm lipid peroxidation. Finally, we used the cryopreserved spermatozoa for in vitro production of embryos. Results showed that combined group of myo‐inositol and melatonin improved the cleavage rate compared to both individual and control groups, although blastocyst rate was improved using both individual and combined groups. In conclusion, co‐supplementation of melatonin and myo‐inositol is a promising approach for the improvement of goat sperm cryopreservation.