A Novel Ratiometric Fluorescent Approach for the Modulation of the Dynamic Range of Lateral Flow Immunoassays. Issue 8 (14th March 2022)

• Record Type: Journal Article
• Authors: Sena‐Torralba, Amadeo; Torné‐Morató, Helena; Parolo, Claudio; Ranjbar, Saba; Farahmand Nejad, Mohammad Amin; Álvarez‐Diduk, Ruslan; Idili, Andrea; Hormozi‐Nezhad, Mohammad Reza; Merkoçi, Arben
• Is Part Of: Advanced materials technologies. Volume 7:Issue 8(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-03-14
• Subjects: fluorescence -- lateral flow assay -- quantum dots -- ratiometry -- sensitivity; Materials science -- Periodicals; Technological innovations -- Periodicals; Materials science; Technological innovations; Periodicals; 620.1105
• DOI: 10.1002/admt.202101450
• Languages: English
• ISSNs: 2365-709X

Abstract

Abstract: The majority of lateral flow assays (LFAs) use single‐color optical labels to provide a qualitative naked‐eye detection, however this detection method displays two important limitations. First, the use of a single‐color label makes the LFA prone to results misinterpretation. Second, it does not allow the precise modulation of the sensitivity and dynamic range of the test. To overcome these limitations, a ratiometric approach is developed. In particular, using anti‐HIgG functionalized red‐fluorescent quantum dots on the conjugate pad (as target dependent labels) and blue‐fluorescent nanoparticles fixed on the test line (as target independent reporters), it is possible to generate a wide color palette (blue, purple, pink, red) on the test line. It is believed that this strategy will facilitate the development of LFAs by easily adjusting their analytical properties to the needs required by the specific application. Abstract : Here the use of target‐dependent and target‐independent fluorescence labels to shift the working range and tune the sensitivity of lateral flow assays are combined. Thanks to this ratiometric approach, both an easier quantification of the test's results by naked eye and a quicker optimization of its analytical performance are achieved.