Differences between cultured cortical neurons by trypsin and papain digestion. Issue 1 (8th March 2022)

• Record Type: Journal Article
• Authors: Hu, Chang‐Yan; Du, Ruo‐Lan; Xiao, Qiu‐Xia; Geng, Min‐Jian
• Is Part Of: Ibrain. Volume 8:Issue 1(2022)
• Page Start: 93
• Page End: 99
• Publication Date: 2022-03-08
• Subjects: cortical neuron -- papain -- trypsin; Brain; Neurosciences; Periodicals; 612.8
• DOI: 10.1002/ibra.12028
• Languages: English
• ISSNs: 2313-1934

Abstract

Abstract: The objective of this study was to compare the efficiency of trypsin and papain in neuronal digestion and determine which enzyme is more efficient. Cortical tissues were obtained from Sprague–Dawley (SD) rats. According to the different digestive enzymes, the samples were divided into the trypsin group and the papain group. After being digested by each of the two enzymes, cortical neurons were collected from the samples. Then, the morphology of the cortical neurons was determined. Moreover, the cortical neurons were transfected with the negative control (NC) lentivirus. The transfection efficiency and morphology were determined and compared. Compared with the papain group, cortical neurons in the trypsin group were more in number, had larger cell size, had longer axonal length, and had fewer impurities. The transfection efficiency of the trypsin group (57.77%) was higher than that of the papain group (53.83%). The morphology of neurons that was displayed showed that the cell body of most neurons shrank and became smaller, and the axis mutation became shorter and less in the papain group 6 days after transfection with the NC lentivirus. Trypsin is more efficient in digesting neurons because the neurons digested by this enzyme are more in number, have a larger cell body, longer axons, and greater transfection efficiency. Abstract : In the present study, a comparative study of trypsin and papain digestion for primary cortical neurons was conducted. Cortical neurons digested by trypsin were more in number, had larger in cell body size, longer in axonal length, and had fewer impurities. Meanwhile, the transfection efficiency of the lentivirus in the trypsin group was higher than that in the papain group. The morphology of neurons that was displayed showed that the cell body of most neurons shrank and became smaller, and the axis mutation became shorter and less in the papain group 6 days after transfection with the NC lentivirus.