Complete genome amplification and sequencing of DENV-2 Pakistani isolates: analysis reveals molecular evolution of DENV-2. (December 2018)

• Record Type: Journal Article
• Authors: Ali, Amjad; Ahmad, Habib; AbouHaidar, Mounir G; Idrees, Muhammad; Ali, Ijaz
• Is Part Of: Future virology. Volume 13:Number 12(2018)
• Page Start: 843
• Page End: 854
• Publication Date: 2018-12
• Subjects: dengue Pakistan -- dengue Swat -- genome sequencing -- PCR; Virology -- Periodicals; Virus diseases -- Treatment -- Periodicals; 616.9101
• DOI: 10.2217/fvl-2018-0149
• Languages: English
• ISSNs: 1746-0794

Abstract

Aim: This study was aimed to amplify, characterize and analyze complete genomes of dengue virus serotype-2 (DENV-2) Pakistani isolates.Patients & methods: Complete genomes of DENV-2 (n = 4) in nine overlapping fragments from two dengue outbreaks (2011 and 2013) were sequenced; deposited in GenBank (Punjab Isolates: KM217157 and KM217156; Swat Isolates: KM217158 and KJ701507) and characterized using specific primers, we designed.Results: Analysis clustered Pakistani isolates with the cosmopolitan genotypes and further indicated that codons 65 of C, 155 of PrM, 203 of E and 254 of NS1 genes were under negative selection pressure. A substitution of alanine with proline in NS5 gene at nucleotide position 885 in Swat isolates was observed.Conclusion: DENV-2 is spreading to other parts of Pakistan with the possible adaptability of the virus molecularly.