Optimization of an ultrasonic-assisted aqueous two-phase extraction method for four flavonoids from Lysionotus pauciflorus. Issue 7 (1st August 2022)

• Record Type: Journal Article
• Authors: Zhou, Hui; Feng, Xiaoye; Yan, Yang; Meng, Xuefei; Wu, Chuanhai; Kang, Yunyao; Li, Yanfang
• Is Part Of: Preparative biochemistry & biotechnology. Volume 52:Issue 7(2022)
• Page Start: 770
• Page End: 782
• Publication Date: 2022-08-01
• Subjects: Aqueous two-phase system -- flavonoids -- Lysionotus pauciflorus -- response surface methodology -- ultrasonic-assisted extraction; Biochemistry -- Technique -- Periodicals; Biotechnology -- Technique -- Periodicals; Biochemistry -- methods -- Periodicals; Biotechnology -- methods -- Periodicals; 660.6
• DOI: 10.1080/10826068.2021.1992783
• Languages: English
• ISSNs: 1082-6068

Abstract

Abstract: An effective approach to extracting four flavonoids (lysioside C, nevadensin-7-sambubioside, ikarisoside B, and nevadensin) from Lysionotus pauciflorus using ultrasonic-assisted aqueous two-phase extraction (UAATPE) technology was studied. Ethanol/K2 HPO4 system was selected and the influence of several important parameters, including composition of the aqueous two-phase system (ATPS), extraction temperature and time, particle size, and solvent to material ratio, were investigated by single factor experimentss. Then three key parameters (ethanol concentration, solvent to material ratio and extraction temperature) were further optimized by response surface methodology (RSM). The optimal process was that 1 g 120 mesh herb powders were extracted with 45 g ATPS (made of 30% ethanol/18% K2 HPO4 ) in 43 °C for 30 min, and yields of four flavonoids could reach 2.56, 2.06, 3.62, and 6.28 mg/g, respectively. Compared with ultrasonic-assisted extraction (UAE) in three conventional solvents (water, ethanol and 60% ethanol), the UAATPE displayed comparatively higher extraction capability. Therefore, UAATPE in ethanol/K2 HPO4 system could be an alternative technology for integrated extraction of the flavonoids from L. pauciflorus .