In cellulo synthesis of dendrimeric sensors for fluorescence-on imaging of bacterial phagocytosis. Issue 25 (11th November 2021)

• Record Type: Journal Article
• Authors: Jiang, Feng; Shi, Yilong; Zou, Xiaoxue; Han, Jiahuai; Han, Shoufa
• Is Part Of: Journal of materials chemistry. Volume 10:Issue 25(2022)
• Page Start: 4800
• Page End: 4809
• Publication Date: 2021-11-11
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d1tb02012a
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Methods for optical tracking of pathogen–host interactions are of biomedical significance. Abstract : Methods for optical tracking of pathogen–host interactions are of biomedical significance. We herein have reported a high molecular weight pH sensor (Den-pH) that is assembled in bacteria and then stably trapped in bacteria irrespective of bacterial membrane potentials. Endowed with acidity-triggered red fluorescence, Den-pH allows signal-on tracking of S. aureus in phagocytosis by macrophages. Intra-bacterial formation of multifunctional optical probes, which offers the advantage of overcoming the liability of conventional potential-sensitive dyes to dissipate from stressed bacteria, offers a new tool to study stressed pathogens.