Allium stipitatum Extract Exhibits In Vivo Antibacterial Activity against Methicillin-Resistant Staphylococcus aureus and Accelerates Burn Wound Healing in a Full-Thickness Murine Burn Model. (22nd February 2017)

• Record Type: Journal Article
• Authors: Karunanidhi, Arunkumar; Ghaznavi-Rad, Ehsanollah; Jeevajothi Nathan, Jayakayatri; Abba, Yusuf; Belkum, Alex van; Neela, Vasanthakumari
• Other Names: Kim Cheorl-Ho Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2017(2017)
• Publication Date: 2017-02-22
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2017/1914732
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : The in vivo antibacterial and burn wound healing potency of Persian shallot bulbs ( Allium stipitatum ) were explored in a mice burn model infected with methicillin-resistant Staphylococcus aureus (MRSA). Hexane (ASHE) and dichloromethane (ASDE) extracts were tested. Female BALB/c mice were inflicted with third-degree thermal injury followed by infection with MRSA. ASHE and ASDE formulated with simple ointment base (SOB) at concentrations of 1%, 2%, and 5% (w/w) were topically applied to burn wounds twice a day for 20 days. Silver sulfadiazine (1%) served as drug positive control. Microbiological analysis was carried out on 1, 2, 3, 4, and 5 days postwounding (dpw) and histopathological analysis at the end of the experiment (20 dpw). Both ointments demonstrated strong antibacterial activity with complete elimination of MRSA at 48–72 h after infection. The rate of wound contraction was higher (95–100%) in mice groups treated with ASHE and ASDE ointments after 15 dpw. Histological analysis revealed significant increase (p < 0.05 ) in epithelialization and collagenation in treated groups. The ASHE and ASDE were found to be relatively noncytotoxic and safe to Vero cell line (383.4 μ g mL −1 ; 390.6 μ g mL −1 ), suggesting the extracts as safe topical antibacterial as well as promising alternatives in managing thermal injuries.