CRISPRedict: a CRISPR-Cas9 web tool for interpretable efficiency predictions. Issue Volume 50:Issue W1(2022) (7th June 2022)

• Record Type: Journal Article
• Authors: Konstantakos, Vasileios; Nentidis, Anastasios; Krithara, Anastasia; Paliouras, Georgios
• Is Part Of: Nucleic acids research. Volume 50:Issue W1(2022)
• Page Start: W191
• Page End: W198
• Publication Date: 2022-06-07
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gkac466
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: The development of the CRISPR-Cas9 technology has provided a simple yet powerful system for genome editing. Current gRNA design tools serve as an important platform for the efficient application of the CRISPR systems. However, most of the existing tools are black-box models that suffer from limitations, such as variable performance and unclear mechanism of decision making. Here, we introduce CRISPRedict, an interpretable gRNA efficiency prediction model for CRISPR-Cas9 gene editing. Its strength lies in the fact that it can accurately predict efficient guide RNAs—with equivalent performance to state-of-the-art tools—while being a simple linear model. Implemented as a user-friendly web server, CRISPRedict offers (i) quick and accurate predictions across various experimental conditions (e.g. U6/T7 transcription); (ii) regression and classification models for scoring gRNAs and (iii) multiple visualizations to explain the obtained results. Given its performance, interpretability, and versatility, we expect that it will assist researchers in the gRNA design process and facilitate genome editing research. CRISPRedict is available for use at http://www.crispredict.org/ . Graphical Abstract: