Profiling and Bioinformatic Analysis of Differentially Expressed Cytokines in Aqueous Humor of High Myopic Eyes – Clues for Anti-VEGF Injections. (2nd January 2020)

• Record Type: Journal Article
• Authors: Zhu, Xiangjia; Du, Yu; Truscott, Roger J.W.; He, Wenwen; Zhou, Peng; Lu, Yi
• Is Part Of: Current eye research. Volume 45:Number 1(2020)
• Page Start: 97
• Page End: 103
• Publication Date: 2020-01-02
• Subjects: High myopia -- aqueous humor -- growth factor -- anti-VEGF injections -- bioinformatic analysis; Ophthalmology -- Periodicals; Eye -- Diseases -- Periodicals; Ophthalmology -- Periodicals; 573.88
• DOI: 10.1080/02713683.2019.1648833
• Languages: English
• ISSNs: 0271-3683

Abstract

ABSTRACT: Purpose: To investigate the aqueous humor growth factor profile in high myopic eyes and analyze the interaction of differentially expressed cytokines. Methods: A case–control study including aqueous humor samples from 36 high myopic patients and 32 controls was conducted. Quantibody® Human Growth Factor Array was used to screen the presence of 40 growth factors in aqueous humor. Expressions of differential growth factors were validated by Bio-Plex Pro TM multiplex bead-based immunoassay. Protein–protein interaction (PPI) and gene ontology (GO) analyses were performed. Results: Growth differentiation factor 15 (GDF-15), hepatocyte growth factor (HGF), and platelet-derived growth factor (PDGF)-AA were found to be significantly higher and vascular endothelial growth factor (VEGF) was detected to be lower in high myopic eyes (all P = .03). Multi-plex bead-based assay further validated the differential expressions of four growth factors and all of them were significantly correlated with axial length ( P < .001). Twenty-six proteins were mapped into PPI network and positive regulation of cell migration, cellular component movement, and cell motility were the most enriched biological processes based on GO analysis. Conclusions: Differential expressed cytokines that indicates a distinctive intraocular microenvironment in high myopic eyes might provide clues for pathological changes within high myopic eyes after anti-VEGF injections.