RelB regulates basal and proinflammatory induction of conjunctival CCL2. (2nd January 2021)

• Record Type: Journal Article
• Authors: Seet, Li-Fong; Toh, Li Zhen; Chu, Stephanie W. L; Wong, Tina T.
• Is Part Of: Ocular immunology and inflammation. Volume 29:Number 1(2021)
• Page Start: 29
• Page End: 42
• Publication Date: 2021-01-02
• Subjects: RelB -- NF-Қb -- CCL2 -- conjunctiva -- inflammation -- surgery; Eye -- Diseases -- Immunological aspects -- Periodicals; Eye -- Inflammation -- Periodicals; 617.7
• DOI: 10.1080/09273948.2019.1662060
• Languages: English
• ISSNs: 0927-3948

Abstract

ABSTRACT: Purpose : This study investigated the involvement of NF-kB in regulating postoperative conjunctival inflammation. Methods : Experimental surgery was performed as described for the mouse model of conjunctival scarring. Expression of NF-κB in postoperative conjunctival tissues or conjunctival fibroblasts were assessed by real-time PCR, immunoblotting and immunofluorescence analyses. Downregulation of RelB was achieved using small interfering RNA. Cellular cytokine secretion was determined using multiplex cytokine assay. Results : RelB was the most highly induced member of the NF-kB family on day 2 post-surgery. Elevated RelB may be found associated with vimentin-positive cells and fibroblasts in vivo and in vitro. In conjunctival fibroblasts, RelB may be induced by TNF-α but not TGF-β2 while its silencing caused selective induction of CCL2 secretion by both basal and TNF-α-stimulated fibroblasts. Conclusions : High RelB induction in the inflammatory phase and the selective modulation of CCL2 suggest a specific anti-inflammatory role for RelB in the postoperative conjunctiva.