Novel strategies for rapid identification and susceptibility testing of MRSA. Issue 8 (2nd August 2020)

• Record Type: Journal Article
• Authors: Mizusawa, Masako; Carroll, Karen C
• Is Part Of: Expert review of anti-infective therapy. Volume 18:Issue 8(2020)
• Page Start: 759
• Page End: 778
• Publication Date: 2020-08-02
• Subjects: mecA -- mecC -- MRSA -- penicillin-binding proteins -- phenotype -- molecular -- resistance -- syndromic panels -- susceptibility testing -- whole-genome sequencing; Anti-infective agents -- Research -- Periodicals; 616.90461
• DOI: 10.1080/14787210.2020.1760842
• Languages: English
• ISSNs: 1478-7210

Abstract

ABSTRACT: Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is associated with adverse clinical outcomes and increased morbidity, mortality, length of hospital stay, and health-care costs. Rapid diagnosis of MRSA infections has been associated with positive impact on clinical outcomes. Areas covered: We searched relevant papers in PubMed for the last 10 years. In major papers, we scanned the bibliographies to ensure that important articles were included. This review describes screening and diagnostic test methods for MRSA and their analytical performances with a focus on rapid molecular-based assays including those that are on the horizon. Future novel technologies will allow more rapid detection of phenotypic resistance. In the case of whole-genome sequencing, detection of mutations may predict resistance, transmission, and virulence. Expert opinion: Currently there are many diagnostic options for the detection of MRSA in surveillance and clinical samples. In general, these are highly accurate and have resulted in improvements in targeted management and reduction in hospital or intensive care unit length of stay for both MSSA and MRSA. Impact on mortality has been variable. Promising novel technologies will not only accurately identify pathogens and detect their resistance markers but will allow discovery of virulence determinants that might further affect patient management.