Therapeutic effects of dexpanthenol on the cardiovascular and respiratory systems following cecal ligation and puncture-induced sepsis in rats. (17th August 2020)

• Record Type: Journal Article
• Authors: Kose, Adem; Parlakpinar, Hakan; Ozhan, Onural; Ermis, Necip; Yildiz, Azibe; Vardi, Nigar; Cigremis, Yilmaz
• Is Part Of: Biotechnic & histochemistry. Volume 95:Number 6(2020)
• Page Start: 428
• Page End: 437
• Publication Date: 2020-08-17
• Subjects: antioxidant -- cecum -- dexpanthenol -- ligation -- puncture -- rat -- sepsis; Histochemistry -- Periodicals; Stains and staining (Microscopy) -- Periodicals; Biotechnology -- Periodicals; 572
• DOI: 10.1080/10520295.2020.1714078
• Languages: English
• ISSNs: 1052-0295

Abstract

ABSTRACT: Cecal ligation and puncture (CLP) is a commonly used model of sepsis in vivo. We investigated the effects of dexpanthenol (DXP) on heart, lung and aorta in CLP-induced sepsis in rats. Rats were divided into four groups of eight: group 1, sham (SH); group 2 (DXP), 500 mg/kg DXP injected intraperitoneally (i.p.); group 3 (CLP), CLP performed; group 4 (CLP + DXP), 500 mg/kg DXP injected i.p. after CLP. Heart, lung and aorta specimens were harvested for histopathological and biochemical analysis. Heart rate increased in group 3 compared to group 1; DXP administration to group 4 did not alleviate this change. In heart tissue samples, MDA levels were decreased significantly in groups 2 and 4 compared to group 3. The levels of GSH in groups 2 and 3 were elevated compared to groups 1 and 2. SOD activity was increased significantly in group 4 compared to group 3. CAT activity for group 4 was increased significantly compared to groups 1 and 3. We found that caspase-9 and caspase-3 activity was increased after application of CLP. Also, DXP treatment decreased the number of caspase-positive cells significantly compared to group 3. DXP appears to be promising for reducing sepsis-related mortality.