Cyclosporin A suppresses Porphyromonas gingivalis lipopolysaccharide induced matrix metalloproteinases activities in the co-culture of human gingival fibroblasts and monocyte cell line THP-1. (3rd March 2020)

• Record Type: Journal Article
• Authors: Jiang, Lei; Liu, Min Ni; Wang, Guo Dong; Wu, Qun; Zhao, Yun Fu
• Is Part Of: Growth factors. Volume 38:Number 2(2020)
• Page Start: 65
• Page End: 74
• Publication Date: 2020-03-03
• Subjects: Cyclosporin A -- cyclophilin A -- CD147 -- matrix metalloproteinases; Growth factors -- Periodicals; Physiology -- Periodicals; 611
• DOI: 10.1080/08977194.2020.1755280
• Languages: English
• ISSNs: 0897-7194

Abstract

Abstract: Cyclosporine-A (CsA) is a widely used immunosuppressant. In this study, we explore the pathway through which CsA suppressed the Porphyromonas gingivalis lipopolysaccharide (P.g-LPS)-induced increase in matrix metalloproteinase (MMP) activities in co-cultured human gingival fibroblasts (HGFs) and THP-1 monocytes. In the co-culture, we found that CsA inhibited the expression of cyclophilin A (CyPA), CD147 and the activities of MMPs, which were all induced by P.g-LPS. We also found that P.g-LPS and recombinant human CyPA increased activation of ERK1/2 and IκB (an NF-κB inhibitory protein), but CsA and the anti-CD147 antibody significantly inhibited these effects. Taken together, CsA in the presence of P.g-LPS might suppress MMP activities by blocking the CyPA/CD147 interaction that results in the inhibition of ERK1/2 and NF-κB signaling by interfering with the phosphorylation of ERK1/2 and IκB.