The impact of ellagic acid on some apoptotic gene expressions: a new perspective for the regulation of pancreatic Nrf-2/NF-κB and Akt/VEGF signaling in CCl4-induced pancreas damage in rats. (4th March 2021)

• Record Type: Journal Article
• Authors: Aslan, Abdullah; Beyaz, Seda; Gok, Ozlem; Can, Muhammed Ismail; Erman, Fazilet; Erman, Orhan
• Is Part Of: Immunopharmacology and immunotoxicology. Volume 43:Number 2(2021)
• Page Start: 145
• Page End: 152
• Publication Date: 2021-03-04
• Subjects: Protein kinase B -- B-cell lymphoma 2 -- ellagic acid -- nuclear factor kappa B -- pancreas damage; Immunopharmacology -- Periodicals; Immunotoxicology -- Periodicals; Antibody-toxin conjugates -- Periodicals; Immunology -- Periodicals; 615.37
• DOI: 10.1080/08923973.2020.1869255
• Languages: English
• ISSNs: 0892-3973

Abstract

Abstract: Objective: The aim of this study was to evaluate the potential effect of ellagic acid (EA) in the treatment of pancreatic injury. EA has been found to have strong anti-inflammatory, antioxidative, and anticancer properties. The effects of EA on pancreati˜c star cell (PSC) activation and cell functions have been evaluated and it has been shown that it inhibits the activation of basic cell functions and PSCs and . it has antidiabetic activity through its effect on β-pancreas cells. Materials and methods: In this work, 36 Wistar albino rats ( n = 36, 8 weeks old) were used. Rats were divided to 4 groups and 9 rats were each group. Groups: Group 1: control group; Group 2: EA group; Group 3: carbon tetrachloride (CCl4 ) group; Group 4: EA + CCl4 group. Animals were decapitated after 8 weeks and their pancreas tissue samples were taken and researched. In pancreas tissue, NF-κB, TNF-α, Nrf-2, VEGF, Bcl-2, caspase-3, and Akt proteins expression ratios were analyzed by western blotting method, CAT activity and GSH levels were determined by spectrophotometer and ROS production was detected by MDA. Results: In our results, the Nrf-2 and caspase-3 protein expressions, catalase activities and GSH levels increased, TNF-α, NF-κB, Bcl-2, VEGF, and Akt protein expressions and MDA levels reduced in EA + CCl4 group comparable to the CCl4 group. Conclusions: These findings reveal that EA decreases pancreas tissue injury in rats and that EA may also be used as a drug against pancreas tissue injury in the future.