Single-stranded DNA versus tailed duplex in sequence conversion of lacZα DNA. (1st September 2020)

• Record Type: Journal Article
• Authors: Kawai, Hidehiko; Sato, Kento; Shirahama, Wataru; Suzuki, Tetsuya; Kamiya, Hiroyuki
• Is Part Of: Nucleosides, nucleotides & nucleic acids. Volume 39:Number 9(2020)
• Page Start: 1245
• Page End: 1250
• Publication Date: 2020-09-01
• Subjects: Sequence conversion -- gene correction -- single-stranded DNA fragment -- 5'-tailed duplex -- genome editing; Nucleosides -- Periodicals; Nucleotides -- Periodicals; Nucleic acids -- Periodicals; 572.8
• DOI: 10.1080/15257770.2020.1790596
• Languages: English
• ISSNs: 1525-7770

Abstract

Abstract: Targeted DNA editing has great potential to cure some genetic diseases; however, the use of artificial nucleases such as CRISPR-Cas9 and TALEN in gene therapy can potentially cause severe side effects due to off-target DNA cleavages. Single-stranded (ss) DNAs and 5'-tailed duplexes (TDs) can achieve target base substitutions when introduced without artificial nucleases into cultured cells and mouse liver. In this study, ss DNA and TD were separately co-introduced into human U2OS cells, together with a target plasmid DNA bearing an inactivated lacZα gene, and the gene correction efficiencies were compared. Unlike the genes examined in previous studies, ss DNA and TD showed similar efficiencies. Therefore, ss DNAs might be as useful as TD for gene correction, depending on the target sequence.