Aqueous angiography in pre‐glaucomatous and glaucomatous ADAMTS10‐mutant canine eyes: A pilot study. (1st November 2021)

• Record Type: Journal Article
• Authors: Burn, Jessica B.; Huang, Alex S.; Weber, Arthur; Komáromy, András M.; Pirie, Christopher G.
• Is Part Of: Veterinary ophthalmology. Volume 25(2022)Supplement 1
• Page Start: 72
• Page End: 83
• Publication Date: 2021-11-01
• Subjects: ADAMTS10 -- aqueous angiography -- canine -- conventional outflow -- indocyanine green -- open‐angle glaucoma; Veterinary ophthalmology -- Periodicals; 636.08977
• DOI: 10.1111/vop.12938
• Languages: English
• ISSNs: 1463-5216

Abstract

Abstract: Objective: To evaluate intravenous scleral and intracameral aqueous angiography in normotensive ( n = 4) and hypertensive glaucomatous ( n = 6) ADAMTS10 ‐mutant canine eyes. Animals studied: Ten ADAMTS10 ‐mutant dogs were used in this study. Procedures: Dogs were sedated and one eye from each dog underwent scleral angiography following intravenous injection of 0.25% indocyanine green (ICG). After a 24‐h recovery period, the same eye underwent aqueous angiography via intracameral administration of ICG. Imaging of identical scleral sectors from the same eye was performed using a Heidelberg Spectralis ® Confocal Scanning Laser Ophthalmoscope. Intrascleral vessel depth and lumen diameters were measured using Heidelberg Spectralis ® optical coherence tomography and computer software. Results: Scleral angiography permitted visualization of vascular components associated with conventional aqueous humor outflow pathways with an average time from injection to fluorescence of 35.8 ± 10.6 s (mean ± SD). Two normotensive eyes (2/10;20%) demonstrated turbulent dye movement, while 4 hypertensive eyes (4/10;40%) exhibited laminar flow. Aqueous angiography demonstrated dye fluorescence within the post‐trabecular conventional aqueous humor outflow pathways in all 10 eyes at 34.3 ± 11.0 s post‐injection. Sectoral and dynamic outflow patterns were observed primarily within the superotemporal sector in nine eyes (9/10; 90%). Seven eyes (7/10; 70%) demonstrated pulsatile dye movement and five eyes (5/10; 50%) exhibited laminar flow. The degree of laminar movement of dye was greatest in hypertensive eyes. Vessel lumen diameters measured 133.85 ± 28.36 µm and 161.18 ± 6.02 µm in hypertensive and normotensive eyes, respectively. Conclusions: Aqueous angiography allowed for visualization of fluorescent dye in the superotemporal sclera. Laminar flow and smaller lumen vessels were observed mainly in hypertensive eyes.