Evaluation of a panel of microsatellite markers to study their applications in Serrapinnus notomelas and to reveal the genetic diversity in Hyphessobrycon eques. Issue 4 (1st August 2022)

• Record Type: Journal Article
• Authors: Yamachita, Andrei Lincoln; Pandolfi, Victor César Freitas; Souza, Felipe Pinheiro de; Godoy, Sara Mataroli de; Lima, Ed Christian Suzuki de; Pereira, Ulisses de Pádua; Povh, Jayme Aparecido; Campos, Eric Costa; Ayres, Denise Rocha; Bignardi, Annaiza Braga; Ruas, Claudete de Fátima; Lopera-Barrero, Nelson Mauricio
• Is Part Of: Animal biotechnology. Volume 33:Issue 4(2022)
• Page Start: 701
• Page End: 709
• Publication Date: 2022-08-01
• Subjects: Genetic conservation -- genetic diversity -- jewel tetra -- molecular marker -- simple sequence repeat; Animal biotechnology -- Periodicals; Animals, Domestic -- genetics -- Periodicals; Animals, Genetically Modified -- Periodicals; 660.6
• DOI: 10.1080/10495398.2020.1826957
• Languages: English
• ISSNs: 1049-5398

Abstract

Abstract: Jewel tetra ( Hyphessobrycon eques ) is a freshwater fish found in several rivers and basins in South America. The present study is the first study to create a panel of microsatellite markers for detecting genetic diversity in H. eques and evaluating the application of these markers in Serrapinnus notomelas . In total, 44 individuals were genotyped from the natural (WIL, n = 20) and stock in captivity (CAP, n = 24) population. Moreover, 19 microsatellite markers were obtained, of which only 8 loci presented a high degree polymorphism. In total, 45 alleles were detected, ranging from 126 bp (Hype2G2) to 420 bp (Hype2E2). The Hardy–Weinberg equilibrium ( p < 0.05) revealed significant difference in one locus in WIL (Hype1G4) and three loci in CAP (Hype1F4, Hype2C3, and Hype2G2). Null alleles ( p < 0.05) were present in only one locus (Hype1G4). The WIL and CAP populations revealed high genetic diversity during FST analysis. The cross-amplification test for S. notomelas revealed that only two loci (Hype2C3 and Hype2G2B) presented satisfactory transferability results. The developed microsatellite primers will be useful in studying the genetic diversity and population structure of H. eques in wild populations and fish farms in the Brazilian and other South American basins.