Angelica Sinensis Polysaccharide Prevents Hematopoietic Stem Cells Senescence in D-Galactose-Induced Aging Mouse Model. (11th April 2017)

• Record Type: Journal Article
• Authors: Mu, Xinyi; Zhang, Yanyan; Li, Jing; Xia, Jieyu; Chen, Xiongbin; Jing, Pengwei; Song, Xiaoying; Wang, Lu; Wang, Yaping
• Other Names: He Tong-Chuan Academic Editor.
• Is Part Of: Stem cells international. Volume 2017(2017)
• Publication Date: 2017-04-11
• Subjects: Stem Cells -- Periodicals; Stem Cells -- Therapeutic use -- Periodicals; Stem Cells -- Transplantation -- Periodicals; 616.0277405
• DOI: 10.1155/2017/3508907
• Languages: English
• ISSNs: 1687-966X

Abstract

Abstract : Age-related regression in hematopoietic stem/progenitor cells (HSC/HPCs) limits replenishment of the blood and immune system and hence contributes to hematopoietic diseases and declined immunity. In this study, we employed D-gal-induced aging mouse model and observed the antiaging effects of Angelica Sinensis Polysaccharide (ASP), a major active ingredient in dong quai (Chinese Angelica Sinensis), on the Sca-1 + HSC/HPCs in vivo. ASP treatment prevents HSC/HPCs senescence with decreased AGEs levels in the serum, reduced SA- β -Gal positive cells, and promoted CFU-Mix formation in the D-gal administrated mouse. We further found that multiple mechanisms were involved: (1) ASP treatment prevented oxidative damage as total antioxidant capacity was increased and levels of reactive oxygen species (ROS), 8-OHdG, and 4-HNE were declined, (2) ASP reduced the expression of γ -H2A.X which is a DNA double strand breaks (DSBs) marker and decreased the subsequent ectopic expressions of effectors in p16 I n k 4 a -RB and p19 A r f -p21 C i p 1 / W a f senescent pathways, and (3) ASP inhibited the excessive activation of Wnt/ β -catenin signaling in aged HSC/HPCs, as the expressions of β -catenin, phospho-GSK-3 β, and TCF-4 were decreased, and the cyto-nuclear translocation of β -catenin was inhibited. Moreover, compared with the positive control of Vitamin E, ASP exhibited a better antiaging effect and a weaker antioxidation ability, suggesting a novel protective role of ASP in the hematopoietic system.