All-in-one disulfide bridging enables the generation of antibody conjugates with modular cargo loading. Issue 30 (20th July 2022)

• Record Type: Journal Article
• Authors: Dannheim, Friederike M.; Walsh, Stephen J.; Orozco, Carolina T.; Hansen, Anders Højgaard; Bargh, Jonathan D.; Jackson, Sophie E.; Bond, Nicholas J.; Parker, Jeremy S.; Carroll, Jason S.; Spring, David R.
• Is Part Of: Chemical science. Volume 13:Issue 30(2022)
• Page Start: 8781
• Page End: 8790
• Publication Date: 2022-07-20
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2sc02198f
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : Tetra-divinylpyrimidine (TetraDVP) linkers offer a method for the generation of antibody conjugates with modular cargo loading and excellent stability via all-in-one disulfide bridging. Abstract : Antibody–drug conjugates (ADCs) are valuable therapeutic entities which leverage the specificity of antibodies to selectively deliver cytotoxins to antigen-expressing targets such as cancer cells. However, current methods for their construction still suffer from a number of shortcomings. For instance, using a single modification technology to modulate the drug-to-antibody ratio (DAR) in integer increments while maintaining homogeneity and stability remains exceptionally challenging. Herein, we report a novel method for the generation of antibody conjugates with modular cargo loading from native antibodies. Our approach relies on a new class of disulfide rebridging linkers, which can react with eight cysteine residues, thereby effecting all-in-one bridging of all four interchain disulfides in an IgG1 antibody with a single linker molecule. Modification of the antibody with the linker in a 1 : 1 ratio enabled the modulation of cargo loading in a quick and selective manner through derivatization of the linker with varying numbers of payload attachment handles to allow for attachment of either 1, 2, 3 or 4 payloads (fluorescent dyes or cytotoxins). Assessment of the biological activity of these conjugates demonstrated their exceptional stability in human plasma and utility for cell-selective cytotoxin delivery or imaging/diagnostic applications.