A perylene diimide-based fluorescent probe for the selective detection of hypochlorite in living cells. (18th July 2022)

• Record Type: Journal Article
• Authors: Zhang, Le; Zhang, Yi-Fan; Han, Ying-Feng
• Is Part Of: Materials chemistry frontiers. Volume 6:Number 16(2022)
• Page Start: 2266
• Page End: 2273
• Publication Date: 2022-07-18
• Subjects: Materials science -- Periodicals; Chemistry -- Periodicals; 540
• DOI: 10.1039/d2qm00283c
• Languages: English
• ISSNs: 2052-1529

Abstract

Abstract : A perylene diimide-based fluorescent probe (PDI-S ) for OCl − detection was developed. PDI-S could sense both the exogenous OCl − and endogenous OCl − with high selectivity and ultra-fast response with high specificity for mitochondria. Abstract : Electron leakage in the respiratory chain of mitochondria is the major causative factor for the production of reactive oxygen species (ROS), including hypochlorite (OCl − ). As one of the most important ROS, OCl − plays a vital role in the innate host defense and regulation of inflammation-related pathological conditions. Herein, a perylene diimide-based fluorescent probe (PDI-S ) for OCl − detection in mitochondria was developed, which utilized the CS bond of imidazole thiones as an OCl − reactive moiety. When PDI-S was reacted with OCl − to form an oxidized cationic diimidazolium salt, a significant enhancement in fluorescence intensity was observed under 564 nm. Cellular imaging revealed that PDI-S could sense both the exogenous OCl − in A549 cells and endogenous OCl − in RAW 264.7 macrophages with high selectivity and ultra-fast response with high specificity for mitochondria. The molecular design represents an attractive paradigm to guide the layout of fluorescent probes for further development of fluorescence detection.