Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer. Issue 16 (17th June 2022)

• Record Type: Journal Article
• Authors: Takahashi, Kazuki; Chida, Shunsuke; Suwatthanarak, Thanawat; Iida, Mikiko; Zhang, Min; Fukuyama, Mao; Maeki, Masatoshi; Ishida, Akihiko; Tani, Hirofumi; Yasui, Takao; Baba, Yoshinobu; Hibara, Akihide; Okochi, Mina; Tokeshi, Manabu
• Is Part Of: Lab on a chip. Volume 22:Issue 16(2022)
• Page Start: 2971
• Page End: 2977
• Publication Date: 2022-06-17
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/d2lc00224h
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : A microfluidic-based non-competitive fluorescence polarization immunoassay using a peptide as a tracer enables simple and rapid CD9 detection. Abstract : This paper is the first report of a non-competitive fluorescence polarization immunoassay (NC-FPIA) using a peptide as a tracer. The NC-FPIA can easily and quickly quantify the target after simply mixing them together. This feature is desirable for point-of-need applications such as clinical diagnostics, infectious disease screening, on-site analysis for food safety, etc. In this study, the NC-FPIA was applied to detect CD9, which is one of the exosome markers. We succeeded in detecting not only CD9 but also CD9 expressing exosomes derived from HeLa cells. This method can be applied to various targets if a tracer for the target can be prepared, and expectations are high for its future uses.