Sperm-like nanocarriers for ultrafast delivery of antisense DNA. Issue 30 (15th July 2022)

• Record Type: Journal Article
• Authors: Tang, Xiaochen; Chen, Tianshu; Chen, Huinan; Yu, Sinuo; Cao, Siyu; Liu, Chenbin; Ma, Yonggeng; Sun, Fenyong; Pan, Qiuhui; Zhu, Xiaoli
• Is Part Of: Nanoscale. Volume 14:Issue 30(2022)
• Page Start: 10844
• Page End: 10850
• Publication Date: 2022-07-15
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d2nr02050e
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : The nano-sperm is developed for ultrafast delivery of antisense DNA into cancer cells. Carried by silver nanoclusters at the "head", antisense DNA as the cargo at the "tail" can be transported into cells rapidly, thereby inhibiting cell proliferation. Abstract : Although various nanomaterials have been designed as intracellular delivery tools, the following aspects have become obstacles to limit their development, like a complex and time-consuming synthesis process, as well as relatively limited application areas ( i.e. biosensing or cell imaging). Here, we developed a novel nano-delivery system called "nano-sperm" with low cytotoxicity and high biocompatibility. In this system, we used DNA oligonucleotides as a backbone to synthesize a nanostructure with silver nanoclusters in the head and functional fragments in the tail, which is shaped like a sperm, to achieve dual functions of ultrafast delivery and imaging/therapy. As a model, we analyzed the possibility of the "nano-sperm" carrying DNA with different structures for imaging or survivin-asDNA for tumor therapy. Therefore, this work reports a novel bifunctional high-speed delivery vehicle, which successfully fills the gap in the field of tumor therapy using DNA-templated nanoclusters as a delivery vehicle.