Altered Innate Immune Responses in Neutrophils from Patients with Well- and Suboptimally Controlled Asthma. (18th November 2015)

• Record Type: Journal Article
• Authors: Tang, Francesca S. M.; Foxley, Gloria J.; Gibson, Peter G.; Burgess, Janette K.; Baines, Katherine J.; Oliver, Brian G.
• Other Names: Lee Kang-Yun Academic Editor.
• Is Part Of: Mediators of inflammation. Volume 2015(2015)
• Publication Date: 2015-11-18
• Subjects: Inflammation -- Mediators -- Periodicals; Biological response modifiers -- Periodicals; Inflammation (Pathologie) -- Médiateurs; Immunomodulateurs; Biological response modifiers; Inflammation -- Mediators; Immunology; Autacoids; Immunologic Factors; Cell Adhesion Molecules; Cell Communication; Cytokines; Inflammation; Periodicals; Electronic journals; 616.0473
• DOI: 10.1155/2015/219374
• Languages: English
• ISSNs: 0962-9351

Abstract

Abstract : Background . Respiratory infections are a major cause of asthma exacerbations where neutrophilic inflammation dominates and is associated with steroid refractory asthma. Structural airway cells in asthma differ from nonasthmatics; however it is unknown if neutrophils differ. We investigated neutrophil immune responses in patients who have good (A G o o d ) and suboptimal (A S u b o p t ) asthma symptom control. Methods . Peripheral blood neutrophils from A G o o d (ACQ < 0.75, n = 11 ), A S u b o p t (ACQ > 0.75, n = 7 ), and healthy controls (HC) (n = 9 ) were stimulated with bacterial (LPS (1 μ g/mL), fMLF (100 nM)), and viral (imiquimod (3 μ g/mL), R848 (1.5 μ g/mL), and poly I:C (10 μ g/mL)) surrogates or live rhinovirus (RV) 16 (MOI1). Cell-free supernatant was collected after 1 h for neutrophil elastase (NE) and matrix metalloproteinase- (MMP-) 9 measurements or after 24 h for CXCL8 release. Results . Constitutive NE was enhanced in A G o o d neutrophils compared to HC. fMLF stimulated neutrophils from A S u b o p t but not A G o o d produced 50% of HC levels. fMLF induced MMP-9 was impaired in A S u b o p t and A G o o d compared to HC. fMLF stimulated CXCL8 but not MMP-9 was positively correlated with FEV1 and FEV1 /FVC. A S u b o p t and A G o o d responded similarly to other stimuli. Conclusions . Circulating neutrophils are different in asthma; however, this is likely to be related to airflow limitation rather than asthma control.