A Two-Piece Derivative of a Group I Intron RNA as a Platform for Designing Self-Assembling RNA Templates to Promote Peptide Ligation. (22nd August 2012)

• Record Type: Journal Article
• Authors: Tanaka, Takahiro; Furuta, Hiroyuki; Ikawa, Yoshiya
• Other Names: Bujnicki Janusz M. Academic Editor.
• Is Part Of: Journal of nucleic acids. Volume 2012(2012)
• Publication Date: 2012-08-22
• Subjects: Nucleic acids -- Periodicals; 572.805
• DOI: 10.1155/2012/305867
• Languages: English
• ISSNs: 2090-0201

Abstract

Abstract : Multicomponent RNA-peptide complexes are attractive from the viewpoint of artificial design of functional biomacromolecular systems. We have developed self-folding and self-assembling RNAs that serve as templates to assist chemical ligation between two reactive peptides with RNA-binding capabilities. The design principle of previous templates, however, can be applied only to limited classes of RNA-binding peptides. In this study, we employed a two-piece derivative of a group I intron RNA from the Tetrahymena large subunit ribosomal RNA (LSU rRNA) as a platform for new template RNAs. In this group I intron-based self-assembling platform, modules for the recognition of substrate peptides can be installed independently from modules holding the platform structure. The new self-assembling platform allows us to expand the repertoire of substrate peptides in template RNA design.