Genomic Profiling of Metastatic Uveal Melanoma Shows Frequent Coexisting BAP1 or SF3B1 and GNAQ/GNA11 Mutations and Correlation With Prognosis. Issue 2 (25th February 2022)

• Record Type: Journal Article
• Authors: Isaacson, Alexandra L; Sompallae, Ramakrishna R; Guseva, Natalya V; Bellizzi, Andrew M; Bossler, Aaron D; Ma, Deqin
• Is Part Of: American journal of clinical pathology. Volume 158:Issue 2(2022)
• Page Start: 177
• Page End: 186
• Publication Date: 2022-02-25
• Subjects: Uveal melanoma -- Genomic profiling -- BAP1 -- SF3B1; Diagnosis, Laboratory -- Periodicals; Pathology -- Periodicals; 616.07
• DOI: 10.1093/ajcp/aqac019
• Languages: English
• ISSNs: 0002-9173

Abstract

Abstract: Objectives: To identify therapeutic targets and correlate with clinical outcomes from mutation profiling of metastatic uveal melanoma (UM) using next-generation sequencing (NGS). Methods: Melanoma cases that were tested using DNA-based NGS panels of 25 and/or 214 genes were evaluated retrospectively (263 cases) and identified 27 UM cases. BAP1 expression was examined by immunohistochemistry. Results: Mutations in GNA11 (14) and GNAQ (12) were found in 96% (n = 27) of cases of UM, and most had coexisting BAP1 (17) or SF3B1 (4) mutations. Coexisting GNAQ/11 - SF3B1 mutations correlated with a longer average time to first metastasis compared with GNAQ/11 - BAP1 mutations (99.7 vs 38.5 months, P = .047). Three patients with BAP1 mutations received trametinib; two are still alive (15 months; 23 months), and one died (32 months). In non-UMs, only 4.2% (n = 236) had BAP1 and 3.8% had SF3B1 mutations; none had coexisting GNAQ/11 mutations. Conclusions: Coexisting BAP1 / SF3B1 and GNAQ/11 mutations were unique to UM. SF3B1 mutations were reported to be UM-specific in melanoma and associated with rare/no metastasis. The finding of mutated SF3B1 in 14.8% (n = 27) of UMs suggests its role should be further evaluated. The correlation of BAP1/SF3B1 mutation with survival also warrants investigation.