Protection and Isolation of Bioorthogonal Metal Catalysts by Using Monolayer‐Coated Nanozymes. (22nd June 2020)

• Record Type: Journal Article
• Authors: Zhang, Xianzhi; Fedeli, Stefano; Gopalakrishnan, Sanjana; Huang, Rui; Gupta, Aarohi; Luther, David C.; Rotello, Vincent M.
• Is Part Of: Chembiochem. Volume 21:Number 19(2020)
• Page Start: 2759
• Page End: 2763
• Publication Date: 2020-06-22
• Subjects: bioorthogonal catalysis -- insulation -- monolayers -- nanozymes -- protection; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202000207
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: We demonstrate here the protection of biorthogonal transition metal catalysts (TMCs) in biological environments by using self‐assembled monolayers on gold nanoparticles (AuNPs). Encapsulation of TMCs in this hydrophobic environment preserves catalytic activity in presence of pH conditions and complex biological media that would deactivate free catalyst. Significantly, the protection affords by these nanozymes extends to isolation of the catalyst active site, as demonstrated by the independence of rate over a wide pH range, in strong contrast to the behavior of the free catalyst. Abstract : On the inside : The catalytic activity of bioorthogonal transition metal catalysts is retained by encapsulating them within the hydrophobic monolayer of gold nanoparticles. This strategy stabilizes the catalyst under environmental conditions, insulates it from changes in pH and prevents degradation over time. This allows for the in situ generation of imaging and therapeutic agents in biological systems.