Coprobacter secundus subsp. similis subsp. nov. and Solibaculum mannosilyticum gen. nov., sp. nov., isolated from human feces. (4th June 2021)

• Record Type: Journal Article
• Authors: Sakamoto, Mitsuo; Ikeyama, Nao; Toyoda, Atsushi; Murakami, Takumi; Mori, Hiroshi; Morohoshi, Sho; Kunihiro, Tadao; Iino, Takao; Ohkuma, Moriya
• Is Part Of: Microbiology and immunology. Volume 65:Number 6(2021)
• Page Start: 245
• Page End: 256
• Publication Date: 2021-06-04
• Subjects: Coprobacter -- human feces -- Solibaculum -- subspecies; Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12886
• Languages: English
• ISSNs: 0385-5600

Abstract

Abstract: An obligately anaerobic, Gram‐stain‐negative, rod‐shaped bacterium, designated strain 2CBH44 T, was isolated from the fecal sample of a healthy Japanese man. This strain was initially assigned as a novel species of the genus Coprobacter based on the 16S rRNA gene sequence similarities compared with other Coprobacter species. The 16S rRNA gene sequence analysis revealed strain 2CBH44 T had relatively low 16S rRNA gene sequence similarity (97.5%) to Coprobacter secundus 177 T . However, strain 2CBH44 T showed 96.9% average nucleotide identity value with C. secundus 177 T, indicating that strain 2CBH44 T and C. secundus 177 T belong to the same species. On the other hand, the digital DNA‐DNA hybridization value between strain 2CBH44 T and C. secundus 177 T was 73.5%, indicating that strain 2CBH44 T is a subspecies of C. secundus . Another anaerobic, Gram‐stain‐variable, rod‐shaped bacterium, designated strain 12CBH8 T, was also isolated from human feces. Strain 12CBH8 T had significantly low 16S rRNA gene sequence similarities (<92.0%) to the validated bacterial species within the family Oscillospiraceae . The percentage of conserved protein values between the genome of strain 12CBH8 T and that of the validated related taxa were <50%, suggesting that strain 12CBH8 T belongs to a novel genus. On the basis of the collected data, strain 2CBH44 T represents a novel subspecies of C. secundus, for which the name Coprobacter secundus subsp. similis subsp. nov. (type strain 2CBH44 T = JCM 34079 T = DSM 111570 T ) is proposed. Strain 12CBH8 T represents a novel species of a novel genus, for which the name Solibaculum mannosilyticum gen. nov., sp. nov. (type strain 12CBH8 T = JCM 34081 T = DSM 111571 T ) is proposed.