Near infrared fluorescent probe for in vivo bioimaging of endogenous hypochlorous acid. (April 2021)

• Record Type: Journal Article
• Authors: Qian, Xiaoli; Yu, Hui; Zhu, Wenchao; Yao, Xufeng; Liu, Wangwang; Yang, Shikui; Zhou, Fangyuan; Liu, Yi
• Is Part Of: Dyes and pigments. Volume 188(2021)
• Publication Date: 2021-04
• Subjects: NIR -- Fluorescent probes -- Hypochlorous acid -- Bioimaging -- In vivo; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2021.109218
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: Accurate detection of HOCl with high sensitivity and high selectivity is essential to elucidate its functions in health or disease states. Herein we propose a new fluorescence HOCl probe (NFL-S) by combining reasonable design strategies and dye screening methods. The probe NFL-S has fast response rate, excellent sensitivity (LOD = 35.0 nM) and outstanding selectivity toward HOCl. It has been successfully applied to detect endogenous HOCl produced by RAW264.7 cells, and further applied to oxidative stress imaging in a mouse arthritis model. Therefore, the probe NFL-S is highly promising to be useful for biological tool to disclose the roles of HOCl in different physiological and pathological contexts. Graphical abstract: Image 1 Highlights: Two NIR sensors (NFL-O and NFL-S) was developed for detecting HOCl. Compared with the inactive molecule (NFL-O), the NFL-S can serve as a highly selective and sensitive sensor. The probes could sensitively response to both exogenous and endogenous HOCl in living cells. The probes could further apply to oxidative stress imaging in a mouse arthritis model.