Decreased serum levels of the inflammaging marker miR-146a are associated with clinical non-response to tocilizumab in COVID-19 patients. (January 2021)

• Record Type: Journal Article
• Authors: Sabbatinelli, Jacopo; Giuliani, Angelica; Matacchione, Giulia; Latini, Silvia; Laprovitera, Noemi; Pomponio, Giovanni; Ferrarini, Alessia; Svegliati Baroni, Silvia; Pavani, Marianna; Moretti, Marco; Gabrielli, Armando; Procopio, Antonio Domenico; Ferracin, Manuela; Bonafè, Massimiliano; Olivieri, Fabiola
• Is Part Of: Mechanisms of ageing and development. Volume 193(2021)
• Publication Date: 2021-01
• Subjects: microRNA -- Inflammaging -- COVID-19 -- Tocilizumab -- interleukin-6; Aging -- Periodicals; Developmental biology -- Periodicals; Aging -- Periodicals; Developmental Biology -- Periodicals; Vieillissement -- Périodiques; Biologie du développement -- Périodiques; Aging; Developmental biology; Periodicals; 612.67
• DOI: 10.1016/j.mad.2020.111413
• Languages: English
• ISSNs: 0047-6374

Abstract

Highlights: Tocilizumab (TCZ) is currently being tested in COVID‐19‐induced cytokine storm. COVID-19 patients responding to TCZ have higher post-treatment levels of circulating miR-146a. Low levels of miR-146a are associated with death in COVID-19 patients not responding to TCZ. MicroRNAs can represent biomarkers of response to anti-inflammatory interventions in COVID-19. Abstract: Current COVID-19 pandemic poses an unprecedented threat to global health and healthcare systems. The most amount of the death toll is accounted by old people affected by age-related diseases that develop a hyper-inflammatory syndrome. In this regard, we hypothesized that COVID-19 severity may be linked to inflammaging. Here, we examined 30 serum samples from patients enrolled in the clinical trial NCT04315480 assessing the clinical response to a single-dose intravenous infusion of the anti-IL-6 receptor drug Tocilizumab (TCZ) in COVID-19 patients with multifocal interstitial pneumonia. In these serum samples, as well as in 29 age- and gender-matched healthy control subjects, we assessed a set of microRNAs that regulate inflammaging, i.e. miR-146a-5p, miR-21-5p, and miR-126-3p, which were quantified by RT-PCR and Droplet Digital PCR. We showed that COVID-19 patients who did not respond to TCZ have lower serum levels of miR-146a-5p after the treatment (p = 0.007). Among non-responders, those with the lowest serum levels of miR-146a-5p experienced the most adverse outcome (p = 0.008). Our data show that a blood-based biomarker, such as miR-146a-5p, can provide clues about the molecular link between inflammaging and COVID-19 clinical course, thus allowing to better understand the use of biologic drug armory against this worldwide health threat.