Bioconversion of syringyl lignin into malic acid by Burkholderia sp. ISTR5. (June 2021)

• Record Type: Journal Article
• Authors: Morya, Raj; Kumar, Madan; Shekhar Thakur, Indu
• Is Part Of: Bioresource technology. Volume 330(2021)
• Publication Date: 2021-06
• Subjects: Syringyl lignin -- Hardwood -- Proteomics -- Genomics -- Malic acid; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.124981
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Syringyl monomeric units were efficiently metabolized by Burkholderia sp. ISTR5. Syringyl lignin bioconversion into malic acid paves the way for efficient biorefinery. Proteogenomic study of R5 on syringyl lignin supported the biovalorization of hardwood lignin. Abstract: Syringyl monomeric units are the most common intermediates encountered during hardwood lignin degradation. In the present study, efficient utilization of syringaldehyde (SAld), syringic acid (SAc) by Burkholderia sp. ISTR5 (R5) has been shown. The proteogenomic analysis of Burkholderia sp. ISTR5 was done to understand the enzymes involved in the degradation of syringaldehyde and syringic acid. Various proteins such as aldehyde dehydrogenase, laccase, and oxidoreductases were highly upregulated during growth on syringaldehyde and syringic acid. R5 completely transformed both the substrates SAld and SAc to other hydrocarbons in 48 h and 24 h, respectively. Moreover, bioconversion of syringyl lignins followed an unusual pathway and accumulated a considerable amount of industrially valuable chemical malic acid in the reaction titer. This study shows the robust chassis of R5 to cope with the aromatic aldehydic stress and simultaneous bioconversion into valuable products for an efficient biorefinery.