Extracellular vesicles derived from mesenchymal cells: perspective treatment for cutaneous wound healing in pediatrics. (9th July 2018)

• Record Type: Journal Article
• Authors: Pelizzo, Gloria; Avanzini, Maria Antonietta; Icaro Cornaglia, Antonia; De Silvestri, Annalisa; Mantelli, Melissa; Travaglino, Paola; Croce, Stefania; Romano, Piero; Avolio, Luigi; Iacob, Giulio; Dominici, Massimo; Calcaterra, Valeria
• Is Part Of: Regenerative medicine. Volume 13:Number 4(2018)
• Page Start: 385
• Page End: 394
• Publication Date: 2018-07-09
• Subjects: animal model -- children -- cutaneous wound repair -- extracellular vesicles -- mesenchymal stem cells; Cellular therapy -- Periodicals; Stem cells -- Transplantation -- Periodicals; Degeneration (Pathology) -- Treatment -- Periodicals; Regeneration (Biology) -- Periodicals; 611.018
• DOI: 10.2217/rme-2018-0001
• Languages: English
• ISSNs: 1746-0751

Abstract

Aim: We evaluated the effects of the intradermal injection of extracellular vesicles (EVs) derived from adipose stem cells (ASC-EVs) and bone marrow cells (BM-EVs) in an experimental cutaneous wound repair model.Methods: Mesenchymal stem cells (MSCs) were in vitro expanded from adipose (ASC) or BM tissues (BM-MSC) of rabbits. EVs were separated from the supernatants of confluent ASC and BM-MSCs. Two skin wounds were induced in each animal and treated with MSC or EV injections. Histological examination was performed postinoculation.Results: EV-treated wounds exhibited a better restoration compared with the counterpart MSC treatment. ASC-EV-treated wounds were significantly better than BM-EVs (p = 0.036).Conclusion: EV topical inoculation provides restored architecture during cutaneous wound healing and represents a promising solution for regenerative medicine in children.