Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy. (27th June 2018)

• Record Type: Journal Article
• Authors: Tickle, Jacqueline A; Poptani, Harish; Taylor, Arthur; Chari, Divya M
• Is Part Of: Nanomedicine. Volume 13:Number 11(2018)
• Page Start: 1333
• Page End: 1348
• Publication Date: 2018-06-27
• Subjects: 3D collagen hydrogel -- astrocytes -- MRI -- transmission electron microscopy; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2017-0347
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To develop a 3D neural cell construct for encapsulated delivery of transplant cells; develop hydrogels seeded with magnetic nanoparticle (MNP)-labeled cells suitable for cell tracking by MRI.Materials & methods: Astrocytes were exogenously labeled with MRI-compatible iron-oxide MNPs prior to intra-construct incorporation within a 3D collagen hydrogel.Results: A connective, complex cellular network was clearly observable within the 3D constructs, with high cellular viability. MNP accumulation in astrocytes provided a hypointense MRI signal at 24 h & 14 days.Conclusion: Our findings support the concept of developing a 3D construct possessing the dual advantages of (i) support of long-term cell survival of neural populations with (ii) the potential for noninvasive MRI-tracking of intra-construct cells for neuroregenerative applications. MNP: Magnetic nanoparticle.