Statistical Optimization of Biobleaching Efficacy of endo-β-1, 4-xylanase from an Actinobacterium Streptomyces olivaceus (MSU3) in Comparison with Zinc Oxide Pretreated Sugarcane Bagasse Pulp Using Box–Behnken Design. Issue 2 (2nd April 2020)
- Record Type:
- Journal Article
- Title:
- Statistical Optimization of Biobleaching Efficacy of endo-β-1, 4-xylanase from an Actinobacterium Streptomyces olivaceus (MSU3) in Comparison with Zinc Oxide Pretreated Sugarcane Bagasse Pulp Using Box–Behnken Design. Issue 2 (2nd April 2020)
- Main Title:
- Statistical Optimization of Biobleaching Efficacy of endo-β-1, 4-xylanase from an Actinobacterium Streptomyces olivaceus (MSU3) in Comparison with Zinc Oxide Pretreated Sugarcane Bagasse Pulp Using Box–Behnken Design
- Authors:
- kumar, Muthusamy Sanjiv
Tamil Selven, Silambarasan
Sholkamy, Essam N.
Mostafa, Ashraf A.
Grasian, Immanuel - Abstract:
- ABSTRACT: The bio-bleaching efficacy of endo-β-1, 4-xylanase from an actinobacterium Streptomyces olivaceus (MSU3) was studied by using sugarcane bagasse pulp in comparison with chemical-mediated bleaching. Before that, the sugarcane bagasse pulp was processed with various concentrations of xylanase (20–100IU) and zinc oxide (0.2–1.0 g) individually through laboratory conditions. Simultaneously, both enzyme- and chemical-mediated bleaching process were optimized through response surface methodology by using the reaction condition containing pH 6.6, temperature 47°C with the incubation time of 3 h. The analysis of variance of the parameters expressed the maximum level of coefficient ( R 2 = 0.963–0.996) with more significant ( P < 0.0001). In bleaching process under-optimized conditions, the highest level of reducing sugar (110.02 mg/g), reduction of kappa number (14.69 k ), degree of brightness (43.62%ISO), level of residual lignin (2.20%) with the discharge of phenolic ( λ 237 = 0.119 OD), and hydrophobic ( λ 465 = 0.046 OD) groups were attained in xylanase (80.0 IU) mediated bleaching than zinc oxide bleaching. Further, the effect of bleaching on processed pulp samples with loosening and changes of surface cell fiber was examined by field emission scanning electron microscopic analysis. The efficient fibrillation and porosity of pulp fiber were seen in xylanase bleached pulp than chemical-bleached pulp. These results clearly indicated that the endo-β-1, 4-xylanase ofABSTRACT: The bio-bleaching efficacy of endo-β-1, 4-xylanase from an actinobacterium Streptomyces olivaceus (MSU3) was studied by using sugarcane bagasse pulp in comparison with chemical-mediated bleaching. Before that, the sugarcane bagasse pulp was processed with various concentrations of xylanase (20–100IU) and zinc oxide (0.2–1.0 g) individually through laboratory conditions. Simultaneously, both enzyme- and chemical-mediated bleaching process were optimized through response surface methodology by using the reaction condition containing pH 6.6, temperature 47°C with the incubation time of 3 h. The analysis of variance of the parameters expressed the maximum level of coefficient ( R 2 = 0.963–0.996) with more significant ( P < 0.0001). In bleaching process under-optimized conditions, the highest level of reducing sugar (110.02 mg/g), reduction of kappa number (14.69 k ), degree of brightness (43.62%ISO), level of residual lignin (2.20%) with the discharge of phenolic ( λ 237 = 0.119 OD), and hydrophobic ( λ 465 = 0.046 OD) groups were attained in xylanase (80.0 IU) mediated bleaching than zinc oxide bleaching. Further, the effect of bleaching on processed pulp samples with loosening and changes of surface cell fiber was examined by field emission scanning electron microscopic analysis. The efficient fibrillation and porosity of pulp fiber were seen in xylanase bleached pulp than chemical-bleached pulp. These results clearly indicated that the endo-β-1, 4-xylanase of the chosen actinobacterium could able to bleach the bagasse pulp effectively than the zinc oxide-mediated bleaching. Graphical abstract: uf0001 … (more)
- Is Part Of:
- Smart science. Volume 8:Issue 2(2020)
- Journal:
- Smart science
- Issue:
- Volume 8:Issue 2(2020)
- Issue Display:
- Volume 8, Issue 2 (2020)
- Year:
- 2020
- Volume:
- 8
- Issue:
- 2
- Issue Sort Value:
- 2020-0008-0002-0000
- Page Start:
- 39
- Page End:
- 49
- Publication Date:
- 2020-04-02
- Subjects:
- Sugarcane bagasse pulp; Streptomyces sp; Xylanase; Zinc oxide; Bleaching
- Journal URLs:
- http://www.tandfonline.com/ ↗
- DOI:
- 10.1080/23080477.2020.1769977 ↗
- Languages:
- English
- ISSNs:
- 2308-0477
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 22874.xml