Transcriptome analysis of two lines of Brassica oleracea in response to early infection with Xanthomonas campestris pv. campestris. Issue 1 (2nd January 2021)

• Record Type: Journal Article
• Authors: Sun, Qingguo; Zhang, Enhui; Liu, Yaping; Xu, Zhongmin; Hui, Maixia; Zhang, Xinxin; Cai, Meijie
• Is Part Of: Canadian journal of plant pathology. Volume 43:Issue 1(2021)
• Page Start: 127
• Page End: 139
• Publication Date: 2021-01-02
• Subjects: black rot -- Brassica oleracea -- disease resistance -- transcriptome -- Xanthomonas campestris pv. campestris; Brassica oleracea -- pourriture noire -- résistance à la maladie -- transcriptome -- Xanthomonas campestris pv. campestris; Plant diseases -- Canada -- Periodicals; Plant diseases -- Periodicals; 632.05
• DOI: 10.1080/07060661.2020.1775705
• Languages: English
• ISSNs: 0706-0661

Abstract

Abstract: Black rot, caused by Xanthomonas campestris pv. campestris (Pammel) Dowson (Xcc), is a devastating disease of cruciferous crops worldwide and a threat to the economic production of Brassica oleracea L. in China. Strategies to control this disease are limited. In addition, knowledge of the molecular mechanisms of pathogenicity and host resistance are still only poorly understood. In this study, we performed transcriptomic analyses of the leaves of two contrasting B. oleracea lines (Xcc-resistant line QP07 and Xcc-susceptible line DBP71) to study the early defence response induced by infection with Xcc. We identified 3357 upregulated and 4091 downregulated genes between QP07 and DBP71 at 0, 12, 24, 48 and 96 h post-inoculation (hpi). A functional annotation pathways analysis of the differentially expressed genes indicated that the glucosinolate biosynthetic and catabolic pathways were enhanced, and ROS scavenging, hormonal, receptor-kinase-related genes and nucleotide binding site (NBS)-encoding resistance genes were expressed during the early infection period. In addition, we found that the host plants may respond to Xcc infection by actively regulating photosynthetic energy metabolism. The results provide new insights to help understand the different responses to Xcc in resistant and susceptible host genotypes, and to aid in the development of effective strategies for the prevention of black rot.