Recent Advances in Synthetic Application and Engineering of Halogenases. Issue 18 (30th July 2020)

• Record Type: Journal Article
• Authors: Minges, Hannah; Sewald, Norbert
• Is Part Of: ChemCatChem. Volume 12:Issue 18(2020)
• Page Start: 4450
• Page End: 4470
• Publication Date: 2020-07-30
• Subjects: biocatalysis -- C−H functionalization -- directed evolution -- halogenase -- protein engineering; Catalysis -- Periodicals; 541.39505
• DOI: 10.1002/cctc.202000531
• Languages: English
• ISSNs: 1867-3880

Abstract

Abstract: Halogenating enzymes are able to introduce halogen substituents under ambient conditions using non‐hazardous reagents with intriguing selectivity, which is highly desired in green chemistry. Although C−H functionalization such as halogenation is a well‐known transformation in synthetic chemistry, the selective incorporation of halogens using conventional chemical approaches often remains challenging. Therefore, enzyme‐based strategies have been emerging as valuable alternatives in recent years. Inspired by manifold developments of enzymatic halogenation, this review focuses on advances of halogenating enzymes and their application with particular emphasis on FAD‐dependent halogenases (FDHs). Catalytic strategies, application scope and engineering of FDHs are outlined pointing to the increasing utility of halogenases as promising biocatalysts. Current limitations as well as potential future developments of their synthetic utility are being discussed. Abstract : Biocatalysis : Halogenases catalyze halogenation of arenes with high regioselectivity under ambient conditions. Still, enzymatic halogenation is considered inefficient, due to insufficient productivity and a limited number of applications reported until now. This review provides an overview of this enzyme class covering structural, mechanistic and enzyme engineering studies. Various examples provide insight into selective biocatalytic C−H functionalization.