OsSYL2AA, an allele identified by gene‐based association, increases style length in rice (Oryza sativa L.). (30th October 2020)

• Record Type: Journal Article
• Authors: Dang, Xiaojing; Yang, Yang; Zhang, Yuanqing; Chen, Xiangong; Fan, Zhilan; Liu, Qiangming; Ji, Jie; Li, Dalu; Li, Yanhui; Fang, Bingjie; Wu, Zexu; Liu, Erbao; Hu, Xiaoxiao; Zhu, Shangshang; She, Dong; Wang, Hui; Li, Yulong; Chen, Siqi; Wu, Yufeng; Hong, Delin
• Is Part Of: Plant journal. Volume 104:Number 6(2020)
• Page Start: 1491
• Page End: 1503
• Publication Date: 2020-10-30
• Subjects: favorable alleles -- genome‐wide association study -- hybrid rice seed production -- natural variation -- stigma characteristics; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.15013
• Languages: English
• ISSNs: 0960-7412

Abstract

SUMMARY: Stigma characteristics are important factors affecting the seed yield of hybrid rice per unit area. Natural variation of stigma characteristics has been reported in rice, but the genetic basis for this variation is largely unknown. We performed a genome‐wide association study on three stigma characteristics in six environments using 1.3 million single‐nucleotide polymorphism (SNPs) characterized in 353 diverse accessions of Oryza sativa . An abundance of phenotypic variation was present in the three stigma characteristics of these collections. We identified four significant SNPs associated with stigma length, 20 SNPs with style length (SYL), and 17 SNPs with the sum of stigma and style length, which were detected repeatedly in more than four environments. Of these SNPs, 28 were novel. We identified two causal gene loci for SYL, OsSYL3 and OsSYL2 ; OsSYL3 was co‐localized with the grain size gene GS3 . The SYL of accessions carrying allele OsSYL3 AA was significantly longer than that of those carrying allele OsSYL3 CC . We also demonstrated that the outcrossing rate of female parents carrying allele OsSYL2 AA increased by 5.71% compared with that of the isogenic line carrying allele OsSYL2 CC in an F1 hybrid seed production field. The allele frequencies of OsSYL3 AA and OsSYL2 AA decreased gradually with an increase in latitude in the Northern Hemisphere. Our results should facilitate the improvement in stigma characteristics of parents of hybrid rice. Significance Statement: The utilization of heterosis in rice is an effective strategy to enhance the grain yield. However, this entails the production of F1 hybrid seeds every year. The yield of F1 hybrid seeds is affected by stigma characteristics. The cloning of OsSYL2 AA filled a gap in the genetic study of style length and could be used for improving stigma characteristics to increase the yield of F1 seeds.