Dye affinity chromatography for fast and simple purification of fucoidan from marine brown algae. Issue 1 (13th October 2015)

• Record Type: Journal Article
• Authors: Hahn, Thomas; Zayed, Ahmed; Kovacheva, Mariya; Stadtmüller, Ralf; Lang, Siegmund; Muffler, Kai; Ulber, Roland
• Is Part Of: Engineering in life sciences. Volume 16:Issue 1(2016)
• Page Start: 78
• Page End: 87
• Publication Date: 2015-10-13
• Subjects: Dye affinity -- Fucoidan -- One‐step purification -- Sepabeads -- Toluidine blue O; Bioengineering -- Periodicals; 660.605
• DOI: 10.1002/elsc.201500044
• Languages: English
• ISSNs: 1618-0240

Abstract

Abstract : Fucoidan is a sulfated polysaccharide with promising pharmacological applications. Due to its medicinal properties, there is a demand for a separation technique that yields a high purification grade. Here, we present a novel purification tool for recovering fucoidan from the marine brown macroalgae Fucus vesiculosus . The developed method is based on amino‐derivatized Sepabeads ® EC‐EA. The beads were modified with toluidine blue (TB), a thiazine derivative, to exploit the strong donor acceptor interactions between the cationic dye and the anionic polysaccharide. The adsorption kinetics and the binding capacity of the resin were analyzed. A Sips model was used to approximate the adsorption isotherm, resulting in a maximum capacity of 127.7 mg fucoidan per g adsorbent. Investigation of the effect of adsorption step's pH on purity and chemical structure was performed by TB and Fourier transform infra‐red spectroscopy assays. Results showed that adsorption at pH 1 and 6 had negligible effects on fucoidan's chemical structure. However, purity was actually improved by 1.55‐ and 1.69‐fold at pH 1 and 6, respectively, with an average yield of 5 g/100 g dried algae powder. In contrast, only a 1.46‐fold increment was observed in fucoidan purified by the traditional method at pH 2, with a yield of 7.5 g/100 g dried algae powder. Furthermore, fucoidan purified by this method at pH 6 complies with, or even exceeds the quality of the commercially available (≥95% pure) fucoidan (Sigma‐Aldrich ® ) with respect to molecular weight and sulfur content. Therefore, dye affinity chromatography provides more advantages than the classically used techniques for fucoidan purification.