MiRNA-20b/SUFU/Wnt axis accelerates gastric cancer cell proliferation, migration and EMT. Issue 4 (April 2021)

• Record Type: Journal Article
• Authors: Peng, Yin; Qin, Ying; Zhang, Xiaojing; Deng, Shiqi; Yuan, Yuan; Feng, Xianling; Chen, Wangchun; Hu, Fan; Gao, Yuli; He, Jieqiong; Cheng, Yulan; Wei, Yanjie; Fan, Xinmin; Ashktorab, Hassan; Smoot, Duane; Li, Song; Meltzer, Stephen J.; Zhuang, Shutong; Tang, Na; Jin, Zhe
• Is Part Of: Heliyon. Volume 7:Issue 4(2021)
• Publication Date: 2021-04
• Subjects: miRNA-20b -- SUFU -- EMT -- Wnt/β-catenin; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2021.e06695
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: Previous research has found that miRNA-20b is highly expressed in gastric cancer (GC), however, its function and underlying mechanism are not clear. Wnt signaling pathway, implicated in tumorigeneisis, is activated in more than 30% of GC. We would like to characterize the biological behavior of miRNA-20b in terms of modulating Wnt/β-catenin signaling and EMT. We showed that miRNA-20b inhibitors suppressed Topflash/Fopflash dependent luciferase activity and the β-catenin nuclear translocation, resulting in inhibition of Wnt pathway activity and EMT. SUFU, negatively regulating Wnt and Hedgehog signaling pathway, was proved to be targeted by miRNA-20b. Moreover, additional knockdown of SUFU alleviated the inhibitory effect on Wnt pathway activity, EMT, cell proliferation/migration and colony formation caused by miRNA-20b inhibition. In summary, miRNA-20b is an oncogenic miRNA and promoted cell proliferation, migration and EMT in GC partially by activating Wnt pathway via targeting SUFU. Abstract : miRNA-20b, SUFU, EMT, Wnt/β-catenin.