Comparative genomics analysis of pTEM-2262, an MDR plasmid from Citrobacter freundii, harboring two unclassified replicons. (November 2018)

• Record Type: Journal Article
• Authors: Li, Manli; Li, Fei; Mi, Zhiqiang; Zhao, Yachao; Zhang, Xianglilan; Jiang, Zhaofang; Pei, Guangqian; Zhou, Lijun; Tong, Yigang; Zhao, Baohua
• Is Part Of: Future microbiology. Volume 13:Number 15(2018)
• Page Start: 1657
• Page End: 1668
• Publication Date: 2018-11
• Subjects: Citrobacter freundii -- mobile elements -- multidrug-resistance -- pTEM-2262 -- unknown incompatibility group; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2018-0243
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: To genetically characterize the multidrug-resistance (MDR) plasmid pTEM-2262 that could not be classified into any known incompatibility group from the clinical Citrobacter freundii isolate 2262.Materials & methods: The repA or repB deletion mutants of pTEM-2262 were constructed using the scarless Cas9-assisted recombineering system. Comparative genomic analysis of pTEM-2262 and the other four previously sequenced plasmids belonging to the same incompatibility group were performed.Results: pTEM-2262, a conjugative plasmid, harbored two unclassified replicons, repA and repB, while repB was not essential for pTEM-2262 replication. In five analyzed plasmids, their conserved backbones primarily integrated massive accessory modules at two 'hotspots' that were located between orf597 and orf504, and between orf393 and orf405 . All the antibiotic resistance genes of pTEM-2262 were clustered in the MDR region with a complex mosaic structure.Conclusion: This study thoroughly investigates the detailed structure and genomic comparison of this unknown incompatibility group for the first time.